Chemical detection of Aflatoxins in dairy products

Chemical detection of Aflatoxins in dairy products

High performance liquid chromatography

High performance liquid chromatography (HPLC) is a widely used analytical technique. It allows samples to be separated and their components determined.

The basic principle of HPLC is passing a sample through a column packed with tiny particles (usually less than 10um diameter) and applying high pressure to increase the resolution and speed of the technique.

There is solvent constantly flowing through the system. This solvent is called mobile phase. To ensure the solvent is constantly moving through the system a pump is employed. This gives the required pressure to allow mobile phase and sample to move through the column with ease and speed. The sample is injected into the mobile phase so it can proceed directly to the column. The sample comes out from the column (elutes) and proceeds to the detector. This monitors the refractive index difference between the pure mobile phase entering the column and the column elute.

The signal from the detector is sent to a data recorder. This measures the time of each component (retention time) and the size of the signal (peak area).
By injecting a known standard a retention time and peak area are achieved. If an unknown sample is then injected and peak obtained at the same retention time.
The peak area can be used to calculate the amount of standard material in the unknown by proportion.

HPLC is especially important for evaluation of non-volatile compounds, providing rapid determination of additives, contaminants and natural components of food products.

Mycotoxins present in food products

Mycotoxins are substances created by fungal secondary metabolic processes which are in connection with specific disorders in animals and humans. The action of toxicity in animals is as harmful as the fungal species which produce these compounds.

Aflatoxins in milk products

A notable example of mycotoxins is aflatoxin that usually brings about liver damage and cancer, reducing the milk production and inhibiting immune system.

Aflatoxin M1, is a toxin found in milk extracted from ingested aflatoxin. Identification of M1 is important since it is likely to be found in milk which is an essential source of nutrition for human.

There are significant regulations (throughout the world) concerning the presence of aflatoxin M1 in dairy products.
Afla M1 HPLC is a rapid aflatoxin test from which accurate numerical results can be achieved. Using monoclonal affinity chromatography, Afla M1 can isolate aflatoxin M1 from dairy products.

Watch the video related to dairy product

becuase it also has omega-3 fatty acids which are essential for your body! * Tofu – This food is added to the list not only because of protein richness, but also its overall health benefits. * Dairy Products – An array of dairy products are low fat or skim milk, yogurts and cheese. These products are great sources of protein & calcium. These will help maintain muscle mass, and strenghten your bones. * Citrus Fruits – Try to get three servings of fruits a day and make some of these citrus …

Help answer the question about dairy product

What would be considered as a dairy product?
Since October after I've gotten sick I haven't been able to sing as great anymore so I went to Hayley Williams of Paramore and she said to try not eating dairy products. But I'm scared to eat anything which left me starving so any sugggestions…

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13 Responses to “Chemical detection of Aflatoxins in dairy products”

  1. mitathehermita says:

    I could listen to you say HORRIBLE over and over and over and over :-p

  2. LyndseyLaceration says:

    You’re amazing.

  3. Trustee says:

    you shouldn't make her. We all have our likes and dislikes. In fact some studies have shown that milk can actually cause osteoporosis. There are plenty of other ways to get the calcium you need (spinach is a great source). If you don't like the coconut milk, try soy or rice milk instead. There are plenty of people who live long happy and healthy lives without drinking milk.

  4. Rachel M says:

    They are considered sweets since they are mainly made of sugar.

  5. melon561 says:

    Soy milk is perfectly safe if you must (or you wish to) avoid dairy. If you don't find a brand of soy milk you like (Silk is best for drinking, but any brand will do for pouring over cereal or baking). If soy doesn't do it for you, try rice milk or almond milk. Keep trying out different brands until you find the one you like.

    You can also have soy ice cream–I'm a fan of Soy Delicious Purely Decadent. There's also rice ice cream, but I haven't tried it yet. Sorbets are suitable, sherbets are not.

    Tofutti makes soy sour cream and soy cream cheese; they make products for the lactose intolerant, but not all of their stuff is vegan.

    If you buy soy cheese, watch out for casein (a dairy protein). There are vegan cheeses out there.

    I'm probably giving you more information than you want, but there's more to dairy than just milk. So do read your labels to ensure there are no milk ingredients in your food.

  6. thepaladin38 says:

    Chocolate can be eaten during pregnancy, especially dark chocolate they say is good for pregnant women… just not too much because of the caffeine. But no, its not a vegetable. She's probably just looking for an excuse to be able to eat it.

  7. marie says:

    tetracycline binds to divalent cations. these are iron, calcium, zinc, etc. (any atom that has a +2 charge).
    the amount of buttermilk would not be a big deal but there are other sources of calcium and iron in their sandwiches. could bind some of the tetracycline.

  8. veggiekila19 says:

    im a veggie killer

  9. Soul of Music! says:

    Avoid milk and anything derived from it (cheese, butter, cream, yoghurt, chocolate)

    You can get a lot of dairy alternatives, it's just a case of finding what you like. There's lots of milk alternatives like soy, rice and almond milk. Keep your eyes out for lactose free stuff, try health food stores. Don't be afraid to eat a tiny bit of dairy by accident, unless you have a very serious intolerance then a little bit won't make you ill.

  10. guildwarsguy123 says:

    Cheese is a good source of calcium, which helps to build strong bones and teeth. Calcium also helps prevent osteoporosis. In 1999, cheese provided 25% of the calcium available in the American food chain, making it an important and readily available source of calcium.

    Cheese also contains a large amount of other essential nutrients such as phosphorous, zinc, riboflavin, vitamin B12 and vitamin A.

    Those of you who have children who are cavity prone might appreciate the fact that many cheeses, including processed American, Bleu, Monterey Jack, Cheddar, and Swiss have been proven to reduce tooth decay. This is in part due to increased saliva production upon eating cheeses, which helps to aid food clearance. Additionally, the milk proteins in cheese help neutralize plaque acids and also help prevent acid demineralization and help remineralization of tooth enamel. For these reasons, it is recommended that cheese be consumed immediately after meals, and as in-between-meal snacks when brushing may not be an option.

    http://www.associatedcontent.com/article/478769/the_surprising_health_benefits_of_cheese.html

  11. GENE says:

    Dairy products are products derived from the mammary glands of mammals. Therefore, eggs are not dairy, unless the laid egg comes out of the chicken's nipple.

    Hope that helps!

  12. Neeneh says:

    You shouldn't have to. My boy was lactose intolerant and I had to stay away from only dairy and he was fine

  13. lastonealiveinside says:

    wtf is that?

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